HTB-177 NCI-H460 人大細胞肺癌細胞

ATCC® Number:  HTB-177™

Designations:  NCI-H460 [H460]

Depositors:   AF Gazdar, JD Minna

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: lung

Disease: carcinoma; large cell lung cancer

Derived from metastatic site: pleural effusion

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:  Isolation date: 1982

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 13

D16S539: 9

D5S818: 9,10

D7S820: 9,12

THO1: 9.3

TPOX: 8

vWA: 17

Cytogenetic Analysis: modal numbr = 57; range = 53 to 65.This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells. Three other markers were found in some cells only. The markers, t(7;9) and t(7;16) were mostly paired. Normal N9 was absent, and N7 and N16 had only a single copy per cell. Two copies each of the X and the Y were present in all cells.

Isoenzymes:  AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1

Gender:  male

Comments: The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung.

The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities.

The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: Twice per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time:  23 hrs in medium with serum; 42 to 60 hrs in serum

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

22434: Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644

32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products

HTB-177 NCI-H460 人大細胞肺癌細胞

MLE-12 小鼠肺上皮細胞

saos-2 人成骨肉瘤細胞

MA104 非洲綠猴胚胎腎上皮細胞

ball-1 細胞

nci-h226 人肺鱗癌細胞

MCA38 小鼠結(jié)腸癌細胞

LTPA 小鼠胰腺癌細胞

NCI-H23 人非小細胞肺癌細胞

293.00     人胚腎細胞

293FT    人胚腎細胞-用于慢病毒包裝

293TN    人胚腎細胞--用于慢病毒包裝

293AV    人胚腎細胞-用于腺病毒包裝

293T    人胚腎細胞

208F    大鼠成纖維細胞

786-O    人腎透明細胞腺癌細胞

A172    人腦膠質(zhì)母細胞瘤細胞

A549    人肺癌細胞 細胞系購買

AGS    人胃癌細胞

BEL7402 人肝癌細胞系

AZ-521    人胃癌細胞

B16F0    小鼠黑色素瘤細胞

Bel-7402    人肝癌細胞

Bel-7404    人肝癌細胞

BGC-823    人胃癌細胞

C6    大鼠腦膠質(zhì)瘤細胞

SW480    人結(jié)腸腺癌細胞

CaCo-2    人結(jié)腸癌細胞

Cho-K1    倉鼠卵巢細胞亞株

COS-7    非洲綠猴SV40轉(zhuǎn)化的腎細胞

GES-1    人胃上皮細胞

HCT-8    人回盲腸癌細胞

HeLa

Hepa1-6    小鼠肝癌細胞

HepG2    人肝癌細胞

hFOB1.19    人永生化成骨細胞

HGC-27    人胃癌細胞

Hs 746T    人胃癌細胞

HT-29    人結(jié)腸癌細胞

KB    人口腔表皮樣癌細胞

LoVo    人結(jié)腸癌細胞

LLC-PK1    豬腎近曲小管上皮細胞

MCF-10A    人正常乳腺上皮細胞

MCF-7    人乳腺癌細胞

MDA-MB-231    人乳腺癌細胞

MGC80-3    人胃癌細胞

MKN-45    人胃癌細胞

NCI-H460    人大細胞肺癌細胞

NIH-3T3    小鼠成纖維細胞

NUGC-3    人胃癌細胞

Raw264-7    小鼠單核細胞白血病細胞

SGC-7901    人胃癌細胞

SMMC-7721    人肝癌細胞

SNU-638    人胃癌細胞

SNU-739    人肝癌細胞

Tca8113    人舌鱗癌細胞    細胞系購買

U251    人腦膠質(zhì)瘤細胞

U-373MG    人腦神經(jīng)膠質(zhì)瘤細胞

U-87 MG    人腦星形膠質(zhì)母細胞瘤

BT-474    人乳腺導(dǎo)管癌細胞

PANC-1    人胰腺癌細胞

HEK293    人胚腎細胞

Hep3B    人肝癌細胞

HL-7702    人正常肝細胞

Raw-Blue    小鼠單核細胞白血病細胞

WEHI-231    小鼠B淋巴細胞

OPM-2    人骨髓瘤細胞

NCI-H929    人骨髓瘤細胞

U266    人骨髓瘤細胞

EJ    人膀胱癌細胞

HOC    大鼠肝卵圓細胞

RWPE-1    人正常前列腺上皮細胞

CAL 27    人舌鱗癌細胞

Calu-3    人肺腺癌細胞

Eca-109    人食管癌細胞

DLD-1    人結(jié)直腸腺癌上皮細胞

5637.00     人膀胱癌細胞

SK-OV-3    人卵巢癌細胞

GBC-SD    人膽囊癌細胞

BEL7402 人肝癌細胞系

SW620    人結(jié)腸癌細胞

MRC-5    人胚肺成纖維細胞     細胞系購買

PC9    人肺癌細胞

PC14    人肺癌細胞

BxPC    人原位胰腺腺癌細胞

PK（15）    豬腎上皮細胞

MDCK    狗腎上皮細胞

3D4/21    豬肺泡巨噬細胞

MHCC-97L    人肝癌細胞（低轉(zhuǎn)移）

MHCC-97H    人肝癌細胞系（高轉(zhuǎn)移）

MiaCaPa-2    人胰腺癌細胞

HN13    口腔鱗狀細胞癌細胞

Bend3    小鼠腦微血管內(nèi)皮細胞

QBC939    人膽管癌細胞

Ishikawa    人子宮內(nèi)膜癌細胞

A498    人腎癌細胞

143B

WEHI-231    小鼠B淋巴細胞

BN

P9R    小鼠骨髓基質(zhì)細胞

P9    小鼠骨髓基質(zhì)細胞

F98    大鼠腦膠質(zhì)瘤細胞

NCIH1299    人非小細胞肺癌細胞

NCIH1650-M3    人非小細胞肺癌細胞

293LVT    人胚腎細胞--用于慢病毒包裝

EC9706    人食管癌細胞

CNE    人鼻咽癌細胞

H322    人非小細胞肺癌細胞

Panc O2

S8    BEL7402 人肝癌細胞系

R5    細胞系購買

Pig MSC    豬骨髓間質(zhì)干細胞

HCAEC    人冠狀動脈內(nèi)皮細胞

marc-145    猴胚胎腎上皮細胞

3D4/21    豬肺泡巨噬細胞

PK（15）    豬腎上皮細胞

MADB-106    大鼠乳腺癌細胞

Sf9    昆蟲卵巢細胞

DADY

HUVEC[HUV-EC-C]    人臍靜脈內(nèi)皮細胞

PC14    人肺癌細胞

PC9    人肺癌細胞

Eca-109    人食管癌細胞

CAL 27    人舌鱗癌細胞

HOC    肝卵圓細胞

EJ    人膀胱癌細胞

U266    人骨髓瘤細胞

293-mTLR5    人胚腎細胞

Raw-Blue    小鼠單核細胞白血病細胞

HL-7702    人正常肝細胞

Hep3B    人肝癌細胞

U-373MG    人腦神經(jīng)膠質(zhì)瘤細胞

Tca-8113    人舌鱗癌細胞

SNU-739    人肝癌細胞

SNU-638    人胃癌細胞

NUGC-3    人胃癌細胞

NIH-3T3    小鼠成纖維細胞

MKN-45    人胃癌細胞

LLC-PK1    豬腎近曲小管上皮細胞

Hs 746T    人胃癌細胞

Hepa1-6    小鼠肝癌細胞

HCT-8    人回盲腸癌細胞

BEL7402 人肝癌細胞系

HaCaT    人永生化表皮細胞

GES-1    人胃上皮細胞

B16-F0    小鼠黑色素瘤細胞

AZ-521    人胃癌細胞

786-O    人腎透明細胞腺癌細胞

208F    大鼠成纖維細胞

293TN    人胚腎細胞--用于慢病毒包裝

293FT    人胚腎細胞-用于慢病毒包裝

GP-293    人胚腎細胞

293-L.P.    人胚腎細胞