CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞的介紹

CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

ATCC® Number: CCL-105™ Price: $323.00

Designations: SW-13

Depositors: A Leibovitz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

Source: Organ: adrenal gland

Tissue: cortex

Tumor Stage: grade IV

Disease: primary small cell carcinoma

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Isolation: Isolation date: August, 1971

Virus Susceptibility: Human poliovirus 1

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile （STR）: Amelogenin: X

CSF1PO: 11,12

D13S317: 9

D16S539: 12

D5S818: 12

D7S820: 8,10

THO1: 7,8

TPOX: 8

vWA: 17,19

Cytogenetic Analysis: modal number = 63; range = 45 to 65.

Approximay 10% of the cells examined possessed a pair of dicentric chromosomes.

Isoenzymes: G6PD, B

Age: 55 years

Gender: female

Ethnicity: Caucasian

Comments: Electron microscopic studies show many bulb gap junctions （BGJ）.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2008

recommended serum:ATCC 30-2020

References: 22140: . . In Vitro 8: 443, 1973.

22526: Leibovitz A, et al. New human cancer cell culture lines. I. SW-13, small-cell carcinoma of the adrenal cortex. J. Natl. Cancer Inst. 51: 691-697, 1973. PubMed: 4765382

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23212: Lasfargues EY, Ozzello L. C*tion of human breast carcinomas. J. Natl. Cancer Inst. 21: 1131-1147, 1958. PubMed: 13611537

26278: Johnson RG, Sheridan JD. Junctions between cancer cells in culture: ultrastructure and permeability. Science 174: 717-719, 1971. PubMed: 4330805

26279: Pinto da Silva P, Gilula NB. Gap junctions in normal and transformed fibroblasts in culture. Exp. Cell Res. 71: 393-401, 1972. PubMed: 4339896

CCL-105