CSF1PO: 6,10

D13S317: 11,13

D16S539: 9,13

D5S818: 10,13

D7S820: 7,12

THO1: 8

TPOX: 8

vWA: 17

The HMy2.CIR cell line was derived from the HMy.2 B lymphoblastoid cell line, a fast growing mutant of the ARH-77 cell line (ATCC CRL-1621), by gamma irradiation and selection for loss of HLA class I antigen expression. [23312]

The cells express no HLA A or B locus products, but do express small amounts of HLA Cw4.

The cells are useful as host for transfected class I major histocompatibility antigen genes.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Every 2 to 3 days

Storage temperature: liquid nitrogen vapor phase

derivative:ATCC CRL-2369

derivative:ATCC CRL-2370

derivative:ATCC CRL-2371